Ayurvedic medicinal plants have been traditionally used for their therapeutic properties, and modern scientific approaches are increasingly being employed to understand their pharmacological potential. This study aimed to comprehensively profile the phytochemical composition of whole extracts from Ayurvedic medicinal plants using liquid chromatography-mass spectrometry (LC-MS). Additionally, an in silico evaluation is planned to be conducted to assess the components of these extracts followed by the physico-chemico-biological evaluations. Furthermore, we aim to perform in vitro and in vivo absorption, distribution, metabolism, and excretion (ADME) characterization and herb-drug interaction (HDI) studies for the whole extracts of Ayurvedic medicinal plants, along with standard-of-care chemotherapeutic drugs used in a known cancer type. This study will provide a platform for evaluation of drugs including in vitro and invitro models. The study will provide valuable insights into the potential therapeutic effects and safety profiles of ayurvedic formulations in presence of chemotherapeutic drugs.