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Electron Microscopy

Electron Microscopy

EM gp
Facility In Charge: Siddhi A. Redkar

The Electron Microscope Facility at ACTREC is equipped with JEM 1400 PLUS, a Transmission electron microscope (JEOL, Japan) functioning at 120kV with magnification up to 12,00,000 X and 0.2nm resolution. TEM analysis provides a detail ultra- structural information of resin embedded or negatively stained biological samples. The facility extends its services for research and analysis of biological samples, nanoparticles and other applications to the external researchers along with the in-house researchers. The facility is also equipped with instruments such as ultra-microtome, glass knife-maker, auto-trimmer and glow discharge unit.

Equipments

TRANSMISSION ELECTRON MICROSCOPE Make: JEOL Model: JEM 1400 PLUS

equipped


JEM 1400 PLUS is a Transmission electron microscope (JEOL, Japan) functioning at 120kV with magnification up to 12,00,000 X and 0.2nm resolution.

Specifications

Accelerating voltage 120 kV
Electron SourceTungsten filament
ResolutionHC pole piece
Point image0.38 nm
Lattice image0.2 nm
Magnificationx200 to x1200000
CameraBottom Mounted Olympus TENGRA 5.3 MP CCD Camera
Tilt range±70°

Applications

  • Imaging of resin embedded cells and tissues.
  • Imaging of negatively stained small particles (<100nm) like bacteriophages, exosomes, nanoparticles, proteins and DNA.
  • Acquisition of immunogold labelled macromolecules to detect specific localization.
  • Elemental detection by Bruker EDS system.

EQUIPMENT: ULTRAMICROTOME Make: Leica Model: UC7

equipped

Ultramicrotome provides high-quality preparation of ultra-thin or semi-thin sections for transmission electron microscopy. The desired thickness of the section can be selected with touchscreen control unit and sections can be cut using glass or diamond knives with motorized knife stage. Majorly, 70nm ultra-thin sections are cut for electron microscopy.

Application

  • Ultra-thin and semi-thin sectioning of resin embedded cells and tissues.

Additional equipment’s

equippedLeica EM TRIM2 auto-trimmer – for trimming the resin blocks,
equippedLeica EM KMR3 – Glass knife maker – for making glass knives.
equippedLeica EM MP Hotplate – for heating the resin sections.

GLOW DISCHARGE UNIT Make: Ted Pella Model: PELCO easiGlow

equipped


The PELCO easiGlow uses an automated and quick cycle with fully selectable parameters for one of the most common glow discharge applications, making TEM support films or grids hydrophilic using air.

Applications

  • To make TEM support films or grids hydrophilic using air
  • Cleaning TEM grids
  • Adhesion and orientation of proteins, nucleic acids and extra cellular vesicles.
  • TEM grid preparation for nanoparticle studies
Current Applications
  • The facility carries out sample preparation for routine electron microscopy, including resin block making (solid tissues, monolayer cell cultures, and cell suspensions) followed by ultrathin sectioning using Leica UC-7 ultramicrotome, contrasting, and acquisition.
  • The facility also carries out negative staining for small particles (<100nm) like bacteriophages, exosomes, nanoparticles, proteins and DNA.
  • The facility provides acquisition of immunogold labelled macromolecules to detect specific localization.
  • The facility provides elemental detection by Bruker EDS system

The major areas of electron microscopy analysis are,

  • Ultrastructure study of cell and tissue,
  • Studying the details at organelle level like mitochondrial changes, ER - mitochondrial interactions, mitochondrial cristae, cell membrane, nuclear architecture, golgi complexes etc.,
  • To check events of autophagy, apoptosis or necroptosis,
  • Characterization of exosomes, bacteriophages and nanoparticles,
  • Uptake and effect of nanoparticles / drugs / radiation on cells or tissue,
  • EDS analysis of nanoparticles
Electron Microscopy ATLAS

Electron Microscopy ATLAS

Sample Submission

External users: Please write to the facility regarding your requirement for electron microscopy. The facility will provide the details as per the analysis requirement. The users are requested to discuss over email regarding their study and sample submission followed by booking a slot accordingly. The samples need to be submitted along with a duly filled requisition form. For quotation, a duly filled requisition form can be mailed to the facility email address (tem.facility@actrec.gov.in). For requisition form, please click the link below,

Internal users (ACTREC):

  • For routine EM, please check with the facility regarding the sample submission requirements as per the study.
  • For negative staining, please book a slot after discussing with the facility for sample submission. Only three samples will be accepted per slot.

    • Samples should be accompanied by a duly filled requisition form and online requisition.

    Requisition form

Charges
ACTREC & DAEOther than ACTREC institutionsCorporate
Routine EM1000500010000
Routine EM + Immunogold labeling1500600012000
Negative staining80040008000

18% GST to be included in addition to the following charges.

Mode of Payment

External users: The users should pay the charges against the quotation or invoice to the following account within a week of the invoice. The users should share the transaction details with the facility once the payment is done. The acquisition will be carried out after the receipt of the payment is confirmed. For e-payment details please click the link below.

Epayment details

Contact

Facility-in-charge:
Siddhi A. Redkar
Technical Officer ‘D’
Electron Microscope Facility
(Khanolkar Shodhika - 68)
ACTREC, TMC
Plot No.1 & 2, Sector 22
Kharghar, Navi Mumbai - 410 210.
022-27405000 / 022-68735000
Extension : 5545
Email : tem.facility@actrec.gov.in

EM Staff:
Ms. Arpana Kadam, Scientific Assistant ‘C’
Mr. Shridhar Nadkar, Technician ‘F’

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