Radiation therapy and chemotherapy are potent tools against cancer, yet they often result in unintended harm to surrounding healthy tissues. This is particularly evident in the gastrointestinal (GI) tract due to its sensitivity to radiation and chemotherapy drugs like Melphalan. Therefore, there is a crucial need to examine the intricate interplay between radiation exposure and chemotherapy treatment on gut health. This study aims to explore the effects of radiation exposure and chemotherapy treatment on physiological parameters in mice, specifically focusing on gut integrity and microbial diversity. Additionally, it seeks to evaluate the potential of phytochemicals like Chlorophyllin, Withaferin etc., commercially available prebiotics such as FOS, and probiotics for example Bacillus clausii as preventive measures for gut toxicity and mitigating microbial dysregulation. Yet in another approach, we aim to study effect of chlorophyllin and natural herbs based sprinkler formulation to enhance the gut health and maintain microbial diversity.